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Genotyping Mice


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Full course description

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MiniCourse Overview

Genotyping is one foundation of mouse research, and this course serves as a helpful resource in your laboratory mouse research.  An essential step in mouse breeding is genotyping mice to ensure that they have the expected genotype.  Why is genotyping so important when working with mice in research, and when should you genotype your mouse?  This course will help you successfully run a JAX protocol covering essential topics related to genotyping JAX mouse strains.

This MiniCourse is self-paced and takes 2 to 3 hours to complete. At the end of the MiniCourse, you will be able to genotype your JAX mouse strain. You will receive a certificate or badge for completing the MiniCourse. In order to claim a certificate or badge for this MiniCourse, you must go through the Core Content module and take the self-review quiz (scoring at least 70%). 

The following topics will be discussed:

  • Importance of genotyping
  • Different types of genotyping strategies
  • Locating JAX protocols
  • JAX protocol information: primer sequences / expected results / reaction components / cycling parameters / specificity
  • Running a JAX protocol
  • Types of primers, reagents, equipment and labware needed
  • DNA preparation
  • Best practices of PCR

Learning Outcomes

By the end of the course, you will be able to:

1. Evaluate the importance of genotyping when working with JAX mice
2. Determine why and when you need to genotype your JAX mice
3. Define different types of genotyping strategies:  standard PCR, melt curve analysis, quantitative PCR and probe/endpoint analysis
4. Find and choose the correct genotyping protocol for your JAX mouse strain
5. Correctly interpret JAX genotyping information
6. Determine the type of primers, reagents, equipment and labware needed to run a genotyping protocol 
7. Prepare DNA from a mouse a for genotyping
8. Apply best practices of PCR

Target Audience

This MiniCourse is designed to meet the needs of people who utilize JAX laboratory mice for genetic and genomic research projects, including: scientists, research assistants, lab technicians, graduate and postdoctoral students, mouse colony managers and staff.

About our Education Offerings

Founded in 1929, The Jackson Laboratory (JAX) is an independent, nonprofit biomedical research institution with more than 2,300 employees who are passionate about our mission: to discover precise genomic solutions for disease and to empower the global biomedical community in the shared quest to improve human health.

While we are not an accredited higher education institution, we offer a variety of education programs — including our non-credit bearing MiniCourses — designed to educate current and future scientists and to provide critical resources, data, tools and services to researchers worldwide.

MiniCourses are created and reviewed by multiple JAX faculty, scientists, educators and other experts, and include interactive content based on the live lectures delivered in our on-campus Courses, Conferences and Workshops.

MiniCourses are available to learners individually and in topical program collections, which are organized around both foundational content and the research areas in which The Jackson Laboratory has earned a reputation for scientific leadership and excellence.

Hardware and Software Requirements

  • Audio speakers or headphones
  • Screen resolution of 800X600 or higher
  • Adobe Reader 5.0 or higher
  • JavaScript enabled
  • 1GB of RAM
  • 2GHz processor

Check the basic computer specifications and supported browsers.  Internet Explorer is not supported.
Should you have questions regarding the content of the MiniCourse or if you need technical support, please email Online and Digital Education at The Jackson Laboratory.