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Cre-lox Technology in Mouse Modeling


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Full course description

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MiniCourse Overview

This MiniCourse is self-paced and takes 3 to 5 hours to complete. At the end of the MiniCourse, you will be able define the unique advantages of using mice in genetic and genomic research. You will receive a certificate and digital badge for completing the MiniCourse. In order to claim a certificate or badge for this MiniCourse, you must go through the Core Content module and take the self-review quiz (scoring at least 70%).

The following topics will be discussed:

  • The P1 bacteriophage viral life cycle
  • The basic Cre-lox system
  • The three Cre-lox mechanisms
  • Breeding schemes for tissue specific, general and inducible knockouts
  • Improving conditional knockout efficiency
  • The FLP-FRT system
  • The Cre reporter strain
  • Overview of the JAX Cre Repository

Learning Outcomes

By the end of the MiniCourse, you will be able to:

  1. Analyze the P1 bacteriophage viral life cycle
  2. Define the basic Cre-lox two component system
  3. Identify the three Cre-lox mechanisms
  4. Comprehend how to generate a tissue-specific knockout strain using the Cre-lox system
  5. Define how to improve conditional knockout efficiency
  6. Comprehend how to generate a germline specific deletion
  7. Comprehend how to generate an inducible Cre mouse model
  8. Explain using the FLP-FRT system with Cre-lox
  9. Define the Cre reporter strain
  10. Appraise current, relevant research highlights
  11. Use Cre-lox technology to model human cancer

Target Audience

This MiniCourse is designed to meet the needs of graduate and post-doctoral students, research assistants, advanced undergraduate students and advanced animal care technicians.

About our Education Offerings

Founded in 1929, The Jackson Laboratory (JAX) is an independent, nonprofit biomedical research institution with more than 2,300 employees who are passionate about our mission: to discover precise genomic solutions for disease and to empower the global biomedical community in the shared quest to improve human health.

While we are not an accredited higher education institution, we offer a variety of education programs — including our non-credit bearing MiniCourses — designed to educate current and future scientists and to provide critical resources, data, tools and services to researchers worldwide.

MiniCourses are created and reviewed by multiple JAX faculty, scientists, educators and other experts, and include interactive content based on the live lectures delivered in our on-campus Courses, Conferences and Workshops.

MiniCourses are available to learners individually and in topical program collections, which are organized around both foundational content and the research areas in which The Jackson Laboratory has earned a reputation for scientific leadership and excellence.

Hardware and Software Requirements

  • Audio speakers or headphones
  • Screen resolution of 800X600 or higher
  • Adobe Reader 5.0 or higher
  • JavaScript enabled
  • 1GB of RAM
  • 2GHz processor

Check the basic computer specifications and supported browsers. Internet Explorer is not supported.

Should you have questions regarding the content of the MiniCourse or if you need technical support, please email Online and Digital Education at The Jackson Laboratory.